| lec # | TOPICS | KEY DATES |
|---|---|---|
| 1 | Course Overview, Enzyme Classification | |
| 2 | Enzyme Catalysts: Review of Protein Chemistry, Enzyme Specificity | |
| 3-4 |
Lecture 3 Non-kinetic Evaluation of Enzyme Performance, Review of Enzyme Kinetics Lecture 4 Enzyme Kinetics (cont.), pH and Temperature Effects, Enzyme Stability |
|
| 5-6 |
Lecture 5 Enzyme Inhibition and Toxicity Lecture 6 Enzyme Inhibition and Toxicity (cont.) |
Lecture 5 |
| 7 | Whole Cell Catalysts: When/Why, Cell Growth vs. Conversion | |
| 8 | Control of Protein Expression (Growth/Non-growth Associated, Catabolite Repression) Toxicity and Inhibition |
|
| 9-11 |
Lecture 9 Engineering Solutions: Acidic/Basic Products, Exothermic Reactions, Enzyme Instability, Equilibrium Reactions Lecture 10 Substrate Solubility, Toxicity Lecture 11 Product Solubility, Toxicity Yield Improvement of Resolutions |
Lecture 9 |
| 12-14 |
Lecture 12 Process Design for Biocatalytic Processes: Purified Enzymes Lecture 13 Process Design (cont.): Whole Cells Lecture 14 Screening for New Catalysts |
|
| 15-17 |
Lecture 15 Improving Catalyst Performance (Biological Solutions): Review of Molecular Biology Lecture 16 Cloning from Whole Cell Catalysts Lecture 17 Site-directed Mutagenesis Directed Evolution of Enzymes |
Lecture 15 Homework #3 due |
| 18 | Presentations of Design/Development Reports |