| WEEK # | TOPICS | READINGS |
|---|---|---|
| 1 |
Introduction of Instructor and Students and Course Overview | |
| 2 |
Antimicrobial Peptides in Innate Immune Defense and Their Production |
M. R., Condon, A. Viera, et al. "Induction of a Rat Enteric Defensin Gene by Hemorrhagic Shock." Infection and Immunity 67, no. 9 (1999): 4787–93. D., Ghosh, E. Porter, et al. "Paneth Cell Trypsin is the Processing Enzyme for Human Defensin-5." Nature Immunology 3, no.6 (2002): 583–90. |
| 3 |
Antimicrobial Peptides as Double Agents |
P. A., Charp, W. G. Rice, et al. "Inhibition of Protein Kinase C by Defensins, Antibiotic Peptides from Human Neutrophils." Biochemical Pharmacology 37, no. 5 (1988): 951–6. F., Navid, M. Boniotto, et al. "Induction of Regulatory T Cells by a Murine β-Defensin." The Journal of Immunology 188, no. 2 (2012): 735–43. |
| 4 |
Folding Matters for Antimicrobial Peptides |
Schroeder B. O., Z. Wu, et al. "Reduction of Disulphide Bonds Unmasks Potent Antimicrobial Activity of Human β-Defensin 1." Nature 469, no. 7330 (2011): 419–23. Z., Wu, D. Hoover, et al. "Engineering Disulfide Bridges to Dissect Antimicrobial and Chemotactic Activities of Human β-Defensin 3." Proceedings of the National Academy of Sciences of the United States of America 100, no. 15 (2003): 8880–5. |
| 5 |
Techniques to Study Antimicrobial Peptides |
Koteva K., H. Hong, et al. "A Vancomycin Photoprobe Identifies the Histidine Kinase VanSsc as a Vancomycin Receptor." Nature Chemical Biology 6, no. 5 (2010): 327–9. Ueberheide, M., A. Fenyö, et al. "Rapid Sensitive Analysis of Cysteine Rich Peptide Venom Components." Proceedings of the National Academy of Sciences of the United States of America 106, no. 17 (2009): 6910–5. |
| 6 |
Peptides in Biotic Interactions |
Haag A. F., M. Baloban, et al. "Protection of Sinorhizobium Against Host Cysteine-Rich Antimicrobial Peptides is Critical for Symbiosis." PLoS Biology 9, no. 10 (2011): e1001169. Cullen T. W., W. B. Schofield, et al. "Antimicrobial Peptide Resistance Mediates Resilience of Prominent Gut Commensals During Inflammation." Science 347, no. 6218 (2015): 170–5. |
| 7 |
Peptide Transporters in Various Organisms |
Y. J., Fei, N. Yoshikatsu, et al. "Expression Cloning of a Mammalian Proton-Coupled Oligopeptide Transporter." Nature 368, no. 6471 (1994): 563–6. G., L. R. Runti, C. S. Maria, et al. "Functional Characterization of SbmA, a Bacterial Inner Membrane Transporter Required for Importing the Antimicrobial Peptide Bac7 (1-35)." Journal of Bacteriology 195, no. 23 (2013): 5343–51. |
| 8 |
Peptide Signals in the Brain |
R., Simantov, and S. H. Snyder. "Morphine-like Peptides in Mammalian Brain: Isolation, Structure Elucidation, and Interactions with the Opiate Receptor." Proceedings of the National Academy of Sciences of the United States of America 73, no. 7 (1976): 2515–9. S. A., Mousa, M. Shakibaei, et al. "Subcellular Pathways of β-Endorphin Synthesis, Processing, and Release from Immunocytes in Inflammatory Pain." Endocrinology 145, no. 3 (2004): 1331–41. |
| 9 |
Molecules of Hunger and Love |
K., Toshinai, H. Yamaguchi, et al. "Des-acyl Ghrelin Induces Food Intake by a Mechanism Independent of the Growth Hormone Secretagogue Receptor." Endocrinology 147, no. 5 (2006): 2306–14. Z., Wang, L. J. Young, et al. "Species Differences in Vasopressin Receptor Binding are Evident Early in Development: Comparative Anatomic Studies in Prairie and Montane Voles." Journal of Comparative Neurology 378, no. 4 (1997): 535–46. |
| 10 |
Epidermal Growth Factors (EGFs) as Signaling Peptides |
S., Felder, K. Miller, et al. "Kinase Activity Controls the Sorting of the Epidermal Growth Factor Receptor Within the Multivesicular Body." Cell 61, no. 4 (1990): 623–34. B., Dawson, B. L. Mitchell, et al. "Epidermal Growth Factor Receptor Dimerization and Activation Require Ligand-induced Conformational Changes in the Dimer Interface." Molecular and Cellular Biology 25, no. 17 (2005): 7734–42. |
| 11 |
Molecules in Action: Antimicrobial Peptides as Drugs |
V., Miao, M. F. Coëffet-Legal, et al. "Daptomycin Biosynthesis in Streptomyces Roseosporus: Cloning and Analysis of the Gene Cluster and Revision of Peptide Stereochemistry." Microbiology 151, no. 5 (2005): 1507–23. J. N., Steenbergen, J. T. Alder, et al. "Daptomycin: A Lipopeptide Antibiotic for the Treatment of Serious Gram-Positive Infections." Journal of Antimicrobial Chemotherapy 55, no. 3 (2005): 283–8. |
| 12 |
Field Trip to Merck Pharmaceuticals | No Readings |
| 13 |
Signaling Peptides as Potential Drugs for Treating Cancers |
J., Chen, X. M. Xu, et al. "Tachyplesin Activates the Classic Complement Pathway to Kill Tumor Cells." Cancer Research 65, no. 11 (2005): 4614–22. T., Kawamoto, J. L. Mendelsohn, et al. "Relation of Epidermal Growth Factor Receptor Concentration to Growth of Human Epidermoid Carcinoma A431 Cells." Journal of Biological Chemistry 259, no. 12 (1984): 7761–6. |
| 14 |
Signaling Peptides in Signal Transduction |
H., Daub, F. U. Weiss, et al. "Role of Transactivation of the EGF Receptor in Signaling by G-Protein-Coupled Receptors." Nature 379, no. 6565 (1996): 557–60. K., Matuoka, M. Shibata, et al. "Cloning of ASH, a Ubiquitous Protein Composed of One Src Homology Region (SH) 2 and Two SH3 Domains, from Human and Rat cDNA Libraries." Proceedings of the National Academy of Sciences of the United States of America 89, no. 19 (1992): 9015–9. |
| 15 |
Last Day of the Class: Oral Presentations, General Discussion of the Course, and Course Evaluations | No Readings |