Readings

This table includes the two required readings for each week, as well as the suggested reading list.

Course readings.
WEEK #	TOPICS	READINGS
1	Introduction See the Lecture Summary	No Readings.
2	Visualization of angiogenic sprouting See the Lecture Summary	Paper 1 Leung, D. W., G. Cachianes, W. J. Kuang, D. V. Goeddel, and N. Ferrara. "Vascular Endothelial Growth Factor is a Secreted Angiogenic Mitogen." Science 246, no. 4935 (December 8, 1989): 1306-9. Paper 2 Gerhardt, H., M. Golding, M. Fruttiger, C. Ruhrberg, A. Lundkvist, A. Abramsson, M. Jeltsch, C. Mitchell, K. Alitalo, D. Shima, and C. Betsholtz. "VEGF Guides Angiogenic Sprouting Utilizing Endothelial Tip Cell Filopodia." J Cell Biol 161, no. 6 (June 23, 2003): 1163-77.
3	Lessons from transgenic mice See the Lecture Summary	Paper 1 Carmeliet, P., V. Ferreira, G. Breier, S. Pollefeyt, L. Kieckens, M. Gertsenstein, M. Fahrig, A. Vandenhoeck, K. Harpal, C. Eberhardt, C. Declercq, J. Pawling, L. Moons, D. Collen, W. Risau, and A. Nagy. "Abnormal Blood Vessel Development and Lethality in Embryos Lacking a Single VEGF Allele." Nature 380, no. 6573 (April 4, 1996): 435-9. Paper 2 Gerety, S. S., and D. J. Anderson. "Cardiovascular EphrinB2 Function is Essential for Embryonic Angiogenesis." Development 129, no. 6 (March, 2002): 1397-410.
4	Intravital imaging of zebrafish See the Lecture Summary	Paper 1 Kamei, M., W. B. Saunders, K. J. Bayless, L. Dye, G. E. Davis, and B. M. Weinstein. "Endothelial Tubes Assemble from Intracellular Vacuoles In Vivo." Nature 442, no. 7101 (July 27, 2006): 453-6. Paper 2 Blum, Y., H. G. Belting, E. Ellertsdottir, L. Herwig, F. Lüders, and M. Affolter. "Complex Cell Rearrangements During Intersegmental Vessel Sprouting and Vessel Fusion in the Zebrafish Embryo." Dev Biol 316, no. 2 (April 15, 2008): 312-22.
5	The importance of pericytes and smooth muscle cells See the Lecture Summary	Paper 1 Uemura, A., M. Ogawa, M. Hirashima, T. Fujiwara, S. Koyama, H. Takagi, Y. Honda, S. J. Wiegand, G. D. Yancopoulos, and S. Nishikawa. "Recombinant Angiopoietin-1 Restores Higher-Order Architecture of Growing Blood Vessels in Mice in the Absence of Mural Cells." J Clin Invest 110, no. 11 (December, 2002): 1619-28. Paper 2 Abraham, S., N. Kogata, R. Fässler, and R. H. Adams. "Integrin β1 Subunit Controls Mural Cell Adhesion, Spreading, and Blood Vessel Wall Stability." Circ Res 102, no. 5 (March 14, 2008): 562-70.
6	Angiogenesis in disease and medicine See the Lecture Summary	Paper 1 Gragoudas, E. S., A. P. Adamis, E. T. Cunningham, Jr., M. Feinsod, and D. R. Guyer. "VEGF Inhibition Study in Ocular Neovascularization Clinical Trial Group." N Engl J Med 351, no. 27 (December 30, 2004): 2805-16. (PDF)^# Paper 2 Hammes, H. P., J. Lin, O. Renner, M. Shani, A. Lundqvist, C. Betsholtz, M. Brownlee, and U. Deutsch. "Pericytes and the Pathogenesis of Diabetic Retinopathy." Diabetes 51, no. 10 (October, 2002): 3107-12.
7	Tumor angiogenesis – a historical perspective See the Lecture Summary	Paper 1 Folkman, J., E. Merler, C. Abernathy, and G. Williams. "Isolation of a Tumor Factor Responsible for Angiogenesis." J Exp Med 133, no. 2 (February 1, 1971): 275-88. Paper 2 Stoletov, K., V. Montel, R. D. Lester, S. L. Gonias, and R. Klemke. "High-Resolution Imaging of the Dynamic Tumor Cell Vascular Interface in Transparent Zebrafish." Proc Natl Acad Sci U S A 104, no. 44 (October 30, 2007): 17406-11.
8	Cutting cancer's supply lines: Targeting the VEGF pathway See the Lecture Summary	Paper 1 Willett, C. G., Y. Boucher, E. Tomaso di., D. G. Duda, L. L. Munn, R. T. Tong, D. C. Chung, D. V. Sahani, S. P. Kalva, S. V. Kozin, M. Mino, K.S. Cohen, D. T. Scadden, A. C. Hartford, A. J. Fischman, J. W.Clark, D. P. Ryan, A. X. Zhu, L. S. Blaszkowsky, H. K. Chen, P. C. Shellito, G. Y. Lauwers, and R. K. Jain. "Direct Evidence that the VEGF-Specific Antibody Bevacizumab has Antivascular Effects in Human Rectal Cancer." Nat Med 10, no. 2 (February, 2004): 145-7. Paper 2 Winkler, F., S. V. Kozin, R. T. Tong, S. S. Chae, M. F. Booth, I. Garkavtsev, L. Xu, D. J. Hicklin, D. Fukumura, E. Tomaso di., L. L. Munn, and R. K. Jain. "Kinetics of Vascular Normalization by VEGFR2 Blockade Governs Brain Tumor Response to Radiation: Role of Oxygenation, Angiopoietin-1, and Matrix Metalloproteinases." Cancer Cell 6, no. 6 (December, 2004): 553-63.
9	Cutting cancer's supply lines: The example of the angiostatin See the Lecture Summary	Paper 1 O'Reilly, M. S., L. Holmgren, Y. Shing, C. Chen, R. A. Rosenthal, M. Moses, W. S. Lane, Y. Cao, E. H. Sage, and J. Folkman. "Angiostatin: A Novel Angiogenesis Inhibitor that Mediates the Suppression of Metastases by a Lewis Lung Carcinoma." Cell 79, no. 2 (October 21, 1994): 315-28. Paper 2 Mauceri, H. J., N. N. Hanna, M. A. Beckett, D. H. Gorski, M. J. Staba, K. A. Stellato, K. Bigelow, R. Heimann, S. Gately, M. Dhanabal, G. A. Soff, V. P. Sukhatme, D. W. Kufe, and R. R. Weichselbaum. "Combined Effects of Angiostatin and Ionizing Radiation in Antitumour Therapy." Nature 394, no. 6690 (July 16, 1998): 287-91.
10	How can anti-angiogenic therapies stimulate rather than inhibit tumor growth and spreading? See the Lecture Summary	Paper 1 Pàez-Ribes, M., E. Allen, J. Hudock, T. Takeda, H. Okuyama, F. Viñals, M. Inoue, G. Bergers, D. Hanahan, and O. Casanovas. "Antiangiogenic Therapy Elicits Malignant Progression of Tumors to Increased Local Invasion and Distant Metastasis." Cancer Cell 15, no. 3 (March 3, 2009): 220-31. Paper 2 Reynolds, A. R., I. R. Hart, A. R. Watson, J. C. Welti, R. G. Silva, S. D. Robinson, G. Da Violante, M. Gourlaouen, M. Salih, M. C. Jones, D. T. Jones, G. Saunders, V. Kostourou, F. Perron-Sierra, J. C. Norman, G. C. Tucker, and K. M. Hodivala-Dilke. "Stimulation of Tumor Growth and Angiogenesis by Low Concentrations of RGD-Mimetic Integrin Inhibitors." Nat Med 15, no. 4 (April, 2009): 392-400. Paper 3 Weller, M., D. Reardon, B. Nabors, R. Stupp. "Will Integrin Inhibitors Have Proangiogenic Effects in the Clinic?" Nat Med 15, no. 7 (July, 2009): 726; author reply 727.
11	Field trip See the Lecture Summary	No Readings.
12	Serendipity! See the Lecture Summary	Paper 1 Ingber, D., T. Fujita, S. Kishimoto, K. Sudo, T. Kanamaru, H. Brem, and J. Folkman. "Synthetic Analogues of Fumagillin that Inhibit Angiogenesis and Suppress Tumour Growth." Nature 348, no. 6301 (December 6, 1990): 555-7. Paper 2 D'Amato, R. J., M. S. Loughnan, E. Flynn, and J. Folkman. "Thalidomide is an Inhibitor of Angiogenesis." Proc Natl Acad Sci U S A 91, no. 9 (April 26, 1994): 4082-5.
13	Oral presentations See the Lecture Summary	No Readings.

Suggested Reading

Carmeliet, P. "Angiogenesis in Health and Disease." Nature Medicine 9, no. 6 (June, 2003): 653-60.

Gaengel, K., G. Genové, A. Armulik, and C. Betsholtz. "Endothelial-Mural Cell Signaling in Vascular Development and Angiogenesis." Arteriosclerosis, Thrombosis, and Vascular Biology 29 (2009): 630-8.

Motiejunaite, R., and A. Kazlauskas. "Pericytes and Ocular Diseases." Experimental Eye Research 86 (2008): 171-7.

Ferrara, N., and R. S. Kerbel. "Angiogenesis as a Therapeutic Target." Nature 438 (December 15, 2005): 967-74.

Ribatti, D. "The History of Angiogenesis Inhibitors." Leukemia 21 (2007): 1606–9.

Neri, D., and R. Bicknell. "Tumour Vascular Targeting." Nature Reviews Cancer, no. 5 (June, 2005): 436-46.

Kerbel, R. S. "Tumor Angiogenesis." N Engl J Med 358 (2008): 2039-49.

Jain, R. K. "Normalization of Tumor Vasculature: An Emerging Concept in Antiangiogenic Therapy." Science 307 (January 7, 2005): 58-62.

Ma, J., and D. J. Waxman. "Combination of Antiangiogenesis with Chemotherapy for More Effective Cancer Treatment." Mol Cancer Ther 7, no. 12 (December, 2008): 3670-84.