| WEEK # | TOPICS | READINGS |
|---|---|---|
| 1 |
Introduction | no readings |
| 2 |
An Introduction to Stem Cells |
Paper 1Martin, G. R. "Isolation of a Pluripotent Cell Line from Early Mouse Embryos Cultured in Medium Conditioned by Teratocarcinoma Stem Cells." Proc Natl Acad Sci USA 78, no. 12 (1981): 7634-8. Paper 2Takahashi, K., and S. Yamanaka. "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126, no. 4 (2006): 663-76. |
| 3 |
Molecular and Cellular Bases of Organ Development |
Paper 1Hyatt, B. A., J. L. Lohr, and H. J. Yost. "Initiation of Vertebrate Left-Right Axis Formation by Maternal Vg1." Nature 384, no. 6604 (1996): 62-5. Paper 2Schuldiner, M., O. Yanuka, J. Eldor-Itskovitz, D. A. Melton, and N. Benvenisty. "Effects of Eight Growth Factors on the Differentiation of Cells Derived from Human Embryonic Stem Cells." Proc Natl Acad Sci USA 97, no. 21 (2000): 11307-12. |
| 4 |
Cloning of Somatic Cells by Nuclear Transfer |
Paper 1McGrath, J., and D. Solter. "Nuclear Transplantation in the Mouse Embryo by Microsurgery and Cell Fusion." Science 220, no. 4603 (1983): 1300-2. Paper 2Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind, and K. H. Campbell. "Viable Offspring Derived from Fetal and Adult Mammalian Cells." Nature 385, no. 6619 (1997): 810-3. |
| 5 |
Therapeutic Uses of Stem Cells |
Paper 1Barnes, D. W., M. J. Corp, J. F. Loutit, and F. E. Neal. "Treatment of Murine Leukaemia with X rays and Homologous Bone Marrow; Preliminary Communication." Br Med J 2, no. 4993 (1956): 626-7. Paper 2Bensinger, W. I., P. J. Martin, B. Storer, R. Clift, S. J. Forman, R. Negrin, A. Kashyap, M. E. Flowers, K. Lilleby, T. R. Chauncey, R. Storb, and F. R. Appelbaum. "Transplantation of Bone Marrow as Compared with Peripheral-Blood Cells from HLA-Identical Relatives in Patients with Hematologic cancers." N Engl J Med 344, no. 3 (2001): 175-81. |
| 6 |
Studies of Patients Treated with Stem Cells |
Paper 1Burt, R. K., Y. Loh, W. Pearce, N. Beohar, W. G. Barr, R. Craig, Y. Wen, J. A. Rapp, and J. Kessler. "Clinical Applications of Blood-Derived and Marrow-Derived Stem Cells for Nonmalignant Diseases." JAMA 299, no. 8 (2008): 925-36. Paper 2Tamaki, H., and M. Matsuoka."Donor-Derived T-cell Leukemia after Bone Marrow Transplantation." N Engl J Med 354, no. 16 (2006): 1758-9. |
| 7 |
Visit to the Massachusetts General Hospital |
Paper 1Adams, G. B., I. R. Alley, U. I. Chung, K. T. Chabner, N. T. Jeanson, C. Lo. Celso, E. S. Marsters, M. Chen, L. S. Weinstein, C. P. Lin, H. M. Kronenberg, and D. T. Scadden. "Haematopoietic Stem Cells Depend on Galpha(s)-Mediated Signalling to Engraft Bone Marrow." Nature 459, no. 7243 (2009): 103-7. Paper 2Fleming, H. E., V. Janzen, C. Lo. Celso, J. Guo, K. M. Leahy, H. M. Kronenberg, and D. T. Scadden. "Wnt Signaling in the Niche Enforces Hematopoietic Stem Cell Quiescence and is Necessary to Preserve Self-Renewal in vivo." Cell Stem Cell 2, no. 3 (2008): 274-83. |
| 8 |
Molecular Bases of Disease |
Paper 1Thomas, J. T., K. Lin, M. Nandedkar, M. Camargo, J. Cervenka, and F. P. Luyten. "A Human Chondrodysplasia Due to a Mutation in a TGF-beta Superfamily Member." Nat Genet 12, no. 3 (1996): 315-7. Paper 2Phelps, R. A., S. Chidester, S. Dehghanizadeh, J. Phelps, I. T. Sandoval, K. Rai, T. Broadbent, S. Sarkar, R. W. Burt, and D. A. Jones. "A Two-Step Model for Colon Adenoma Initiation and Progression Caused by APC Loss." Cell 137, no. 4 (2009): 623-34. |
| 9 |
In vivo Regeneration of Tissues by Cell Transplantation |
Paper 1Lee, K. B., J. H. Hui, I. C. Song, L. Ardany, and E. H. Lee. "Injectable Mesenchymal Stem Cell Therapy for Large Cartilage Defects—a Porcine Model." Stem Cells 25, no. 11 (2007): 2964-71. Paper 2Strauer, B. E., M. Brehm, T. Zeus, M. Köstering, A. Hernandez, R. V. Sorg, G. Kögler, and P. Wernet. "Repair of Infarcted Myocardium by Autologous Intracoronary Mononuclear Bone Marrow Cell Transplantation in Humans." Circulation 106 (2002): 1913-1918. |
| 10 |
IPS Cells as Experimental Models of Neurological Disorders |
Paper 1Ebert, A. D., J. Yu , F. F. Rose Jr., V. B. Mattis, C. L. Lorson, J. A. Thomson, C. N. Svendsen. "Induced Pluripotent Stem Cells from a Spinal Muscular Atrophy Patient." Nature 457, no. 7227 (2009): 277-80. Paper 2Ohtaki H., J. H. Ylostalo, J. E. Foraker, A. P. Robinson, R. L. Reger, S. Shioda, and D. J. Prockop. "Stem/Progenitor Cells from Bone Marrow Decrease Neuronal Death in Global Ischemia by Modulation of Inflammatory/immune Responses." Proc Natl Acad Sci USA 105, no. 38 (2008): 14638-43. |
| 11 |
Bioartificial Organs |
Paper 1Ott, H. C., T. S. Matthiesen, S. K. Goh, L. D. Black, S. M. Kren, T. I. Netoff, and D. A. Taylor. "Perfusion-Decellularized Matrix: Using Nature's Platform to Engineer a Bioartificial Heart." Nat Med 14, no. 2 (2008): 213-21. Paper 2Humes, H. D., D. A. Buffington, S. M. MacKay, A. J. Funke, and W. F. Weitzel. "Replacement of Renal Function in Uremic Animals with a Tissue-Engineered Kidney." Nat Biotechnol 17, no. 5 (1999): 451-5. |
| 12 |
Tissue Regeneration Driven by Growth Hormones |
Paper 1Urist, M. R. "Bone: Formation by Autoinduction." Science 150 (1965): 893-9. Paper 2Friedlaender, G. E., C. R. Perry, J. D. Cole, S. D. Cook, G. Cierny, G. F. Muschler, G. A. Zych, J. H. Calhoun, A. J. LaForte, and S. Yin. "Osteogenic Protein-1 (bone morphogenetic protein-7) in the Treatment of Tibial Nonunions." J Bone Joint Surg Am 83-A, no. Suppl 1(Pt 2) (2001): S151-8. |
| 13 |
Field Trip to Genzyme | no readings |
| 14 |
Student Paper Presentations and Discussions | no readings |