Session Overview
This session will cover 1) what restriction enzymes are and how they cut DNA, 2) the different types of restriction enzymes and the advantages and disadvantages of using them, and 3) how restriction enzymes are used to create a recombinant DNA molecule. Learning Objectives
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Session Activities
Lecture Video
Watch the lecture video excerpt
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Basic Mechanisms of Cloning, Excerpt 1 (00:13:19)
Basic Mechanisms of Cloning, Excerpt 1
> Download from iTunes U (MP4 - 29.2MB)
> Download from Internet Archive (MP4 - 29.2MB)
Check Yourself
Session Activities
Lecture Video
Watch the lecture video excerpt
-
Basic Mechanisms of Cloning, Excerpt 2 (00:13:01)
Basic Mechanisms of Cloning, Excerpt 2
> Download from iTunes U (MP4 - 28.5MB)
> Download from Internet Archive (MP4 - 28.5MB)
Check Yourself
Session Activities
Lecture Video
Watch the lecture video excerpt
-
Basic Mechanisms of Cloning, Excerpt 3 (00:14:09)
Basic Mechanisms of Cloning, Excerpt 3
> Download from iTunes U (MP4 - 31MB)
> Download from Internet Archive (MP4 - 31MB)
Check Yourself
You want to:
- Obtain the human insulin gene.
- Cut chosen vector and ligate the human insulin gene into vector.
- Use this ligation mixture to transform E. coli cells.
- Transfer E. coli cells to growth media.
- Select for E. coli cells that have obtained any vector.
Each of the steps listed above requires a specific DNA sequence found on the vector.
Session Activities
Practice Problems
Further Study
Suggested topics for further study in an introductory-level Biology textbook
- Steps involved in a basic cloning strategy
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